Marijuana and Cannabinoid Research: Methods and Protocols

Emmanuel S. Onaivi1588293505, 9781588293503

A cutting-edge collection of readily reproducible in vitro and in vivo methods to elucidate the mechanisms associated with cannabinoid function in health and disease. The techniques can be used in studies across the board from genes to behavior. The molecular neurobiological methods are invaluable in analyzing the structure, the polymorphisms, and the molecular expression of the cannabinoid receptors (CBrs), as well as their association with polysubstance abuse. There are also methods for localizing cannabinoid receptors in different systems, visualizing cannabinoid effects using brain slice imaging and elctrophysiological approaches, and designing and synthesizing cannabinoids and endocannabinoids. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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Table of contents :
01cover.pdf……Page 1
02content.pdf……Page 2
999-0-1-18.pdf……Page 18
999-0-19-40.pdf……Page 36
999-0-41-70.pdf……Page 58
999-0-71-90.pdf……Page 88
999-0-91-104.pdf……Page 108
999-0-105-112.pdf……Page 122
999-0-113-148.pdf……Page 130
999-0-149-162.pdf……Page 166
999-0-163-168.pdf……Page 180
999-0-169-190.pdf……Page 186
169-190_Onaivi_10.pdf……Page 0
69……Page 196
999-0-191-208.pdf……Page 208
999-0-209-216.pdf……Page 226
999-0-217-234.pdf……Page 234
999-0-235-254.pdf……Page 252
999-0-255-268.pdf……Page 272
999-0-269-290.pdf……Page 286
999-0-291-298.pdf……Page 308
index.pdf……Page 316