Applications of HPLC in Biochemistry

A. Fallon, R.F.G. Booth and L.D. Bell (Eds.)0444808620, 9780444808622

This book is intended to familiarize biochemists with HPLC. Theoretical aspects of each mode of chromatography are discussed in chapters 1-9, providing an understanding of the various modes of chromatography which are now possible using commercially available columns, from reversed phase to affinity. Practical aspects and instrumentation are covered in chapter 10. The bulk of the book, which follows, presents examples and applications of each mode of chromatography in current biochemical practice.

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Table of contents :
Content:
Advisory board
Page ii

Edited by
Page iii

Copyright page
Page iv

Acknowledgements
Page vi

Chapter 1 The Origins and development of liquid chromatography
Pages 1-7

Chapter 2 The theory of HPLC
Pages 8-22

Chapter 3 Instrumentation
Pages 23-42

Chapter 4 High Performance Ion-Exchange Chromatography
Pages 43-55

Chapter 5 High performance size exclusion chromatography
Pages 56-64

Chapter 6 High performance normal phase chromatography
Pages 65-72

Chapter 7 High performance reversed phase chromatography
Pages 73-89

Chapter 8 High performance reverse phase ion-pair chromatography
Pages 90-101

Chapter 9 High performance affinity chromatography
Pages 102-114

Chapter 10 Practical aspects
Pages 115-142

Chapter 11.1 Nucleosides and nucleotides
Pages 144-168

Chapter 11.2 Proteins, peptides and amino acids
Pages 169-192

Chapter 11.3 Lipids
Pages 193-212

Chapter 11.4 Carbohydrates
Pages 213-229

Chapter 11.5 Prostaglandins, leukotrienes and hydroxyeicosatetraenoic acids
Pages 230-243

Chapter 11.6 Steroids
Pages 244-259

Chapter 11.7 Biogenic amines
Pages 260-270

Chapter 11.8 Vitamins
Pages 271-294

Chapter 11.9 Antibiotics
Pages 295-305

Appendix
Pages 306-311

References
Pages 312-331

Subject index
Pages 332-338