Electron tomography: methods for three-dimensional visualization of structures in the cell

Joachim Frank038731234X, 9780387312347

Electron tomography has become a standard technique with applications in cell biology, structural biology, and materials science. This definitive work provides a comprehensive treatment of the mathematical background and working methods of three-dimensional reconstruction from tilt series, with special emphasis on the problems presented by limitations of data collection in the transmission electron microscope. In addition to chapters that are applicable to 3D reconstruction in all fields of science, such as radiological imaging in medicine and electron tomography in materials science, Electron Tomography also focuses on specimen preparation and imaging unique to biological electron microscopy.

This extensively revised second edition updates key contributions on the mathematics of 3D reconstruction, and includes new topics such as automated tomography, frozen sectioning of cells, and the interpretation of density maps through methods of fitting, docking, denoising, and segmentation. Each chapter is a self-contained treatise by a world expert in the author’s field of research, resulting in an indispensable resource and companion for laboratories that practice electron tomography or seek to implement electron tomography as a tool for visualization of cells and cell components.

 Key Features
Presents the mathematical background and working methods for three-dimensional reconstruction from projections Takes the reader from biological specimen preparation to three-dimensional images of the cell and its components Revised and updated extensively from the first edition published in 1992 The definitive work in the field written by leading international experts

---

Table of contents :
cover-large.gif.jpg……Page 1
1.pdf……Page 2
2.pdf……Page 13
3.pdf……Page 28
4.pdf……Page 60
5.pdf……Page 93
6.pdf……Page 122
7.pdf……Page 171
8.pdf……Page 194
9.pdf……Page 223
10.pdf……Page 250
11.pdf……Page 279
12.pdf……Page 310
13.pdf……Page 334
14.pdf……Page 356
15.pdf……Page 374
16.pdf……Page 403
17.pdf……Page 419
18.pdf……Page 442