Laboratory Manual and Workbook in Microbiology: Applications to Patient Care

Josephine A. Morello, Paul A Granato, Helen Eckel Mizer9780072463545, 0072463546

This microbiology laboratory manual is designed especially for the non-majors, health science microbiology courses. The organization reflects the body systems approach and contains specific sections on clinical diagnosis. 36 exercises and 43 experiments cover a broad range of topics.

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Table of contents :
Cover……Page 1
Preface……Page 2
1. Orientation to the Microbiology Laboratory……Page 5
The Microscope……Page 8
Handling and Examining Cultures……Page 17
2. Microscopic Morphology of Microorganisms……Page 27
Hanging-Drop and Wet-Mount Preparations……Page 28
Simple Stains……Page 35
3. Differential Stains……Page 39
Gram Stain……Page 40
Acid-Fast Stain……Page 45
Special Stains……Page 49
4. Cultivation of Microorganisms……Page 55
Culture Media……Page 56
Streaking Technique to Obtain Pure Cultures……Page 61
Pour-Plate and Subculture Techniques……Page 67
Culturing Microorganisms from the Environment……Page 71
5. Physical Antimicrobial Agents……Page 75
Moist and Dry Heat……Page 76
The Autoclave……Page 81
6. Chemical Antimicrobial Agents……Page 88
Disinfectants……Page 89
Antimicrobial Agent Susceptibility Testing and Resistance……Page 94
7. Principles of Diagnostic Biology……Page 105
Primary Media for Isolation of Microorganisms……Page 106
Some Metabolic Activities of Bacteria……Page 111
Activities of Bacterial Enzymes……Page 117
Principles of Antigen Detection and Nucleic Acid Assays for Detection and Identification of Microorganisms……Page 125
8. Microbiology of the Respiratory Tract……Page 135
Staphylococci……Page 136
Streptococci, Pneumococci, and Enterococci……Page 143
Haemophilus, Corynebacteria, and Bordetella……Page 159
Clinical Specimens from the Respiratory Tract……Page 169
9. Microbiology of the Intestinal Tract……Page 176
The Enterobacteriaceae (Enteric Bacilli)……Page 177
Clinical Specimens from the Intestinal Tract……Page 192
10. Microbiology of the Urinary and Genital Tracts……Page 198
Urine Culture Techniques……Page 199
Neisseria and Spirochetes……Page 206
11. Microbial Pathogens Requiring Special Laboratory Techniques……Page 214
Anaerobic Bacteria……Page 215
Mycobacteria……Page 224
Mycoplasmas, Rickettsiae, Chlamydiae, Viruses, and Prions……Page 229
Fungi: Yeasts and Molds……Page 240
Protozoa and Animal Parasites……Page 250
Serological Identification of Patients’ Antibodies……Page 260
IV. Applied (Sanitary) Microbiology……Page 263
Bacteriological Analysis of Water……Page 264
Bacteriological Analysis of Milk……Page 267
Selected literature……Page 271
Index……Page 272
Colour plates……Page 278