Stability and Stabilization of Biocatalysts, Proceedings of an International Symposium organized under auspices of the Working Party on Applied Biocatalysis of the European Federation of Biotechnology, the University of Cordoba, Spain, and the Spanish Society of Biotechnology

A. Ballesteros, F.J. Plou, J.L. Iborra and P.J. Halling (Eds.)0-444-82970-9, 9780444829702

Six years after the symposium on Stability and Stabilization of Enzymes, a second symposium, Stability and Stabilization of Biocatalysts, on which this book is based, was organized. At the symposium, 210 participants representing all continents came together to learn from 150 oral and poster communications.The volume brings up-to-date the work already going on, and identifies possible breakthroughs in the research. This timely book therefore presents cutting edge developments in topics such as non-covalent processes in solution, protein engineering and thermophile enzymes, immobilized enzymes, non-conventional media, and whole cells.

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Table of contents :
Content:
Preface
Page v

Acknowledgements
Page vii

Diagnosing the inactivating process of enzymes Original Research Article
Pages 3-18
Onno Misset, Alard van Dijk

Stability and stabilization of glucose-fructose oxidoreductase from Zymomonas mobilis against irreversible inactivation during substrate turnover in biochemical reactors Original Research Article
Pages 19-26
B. Nidetzky, M. Fürlinger, D. Haltrich, K.D. Kulbe

Reactor performance under thermal inactivation and temperature optimization with chitin-immobilized lactase Original Research Article
Pages 27-34
A. Illanes, L. Wilson, C. Altamirano, A. Aillapán

Fast in-situ-characterisation of biocatalysts long-term stability Original Research Article
Pages 35-40
M. Boy, A. Dominik, H. Voss

Chemical and thermal stability of ferulic acid (feruloyl) esterases from Aspergillus Original Research Article
Pages 41-46
C.B. Faulds, F.O. Aliwan, R.P. de Vries, R.W. Pickersgill, J. Visser, G. Williamson

Stability properties of calf intestinal alkaline phosphatase Original Research Article
Pages 47-52
Ultan McKeon, Brendan O’Connor, Ciarán Ó’Fágáin

Stabilization of β-glucanase and its effect on the substrate hydrolysis pattern Original Research Article
Pages 53-58
I. Marković, B. Marković-Devčić, S. Gamulin, N. Pavlović

Some factors affecting the behavior of anhydrous α-chymotrypsin at high temperature Original Research Article
Pages 59-64
Domenico Pirozzi Guido Greco jr.

Horseradish peroxidase stability in the course of phenols oxidation Original Research Article
Pages 65-70
A.M. Egorov, Yu.L. Kapeluich, T.A. Pastuhova, M.Yu. Rubtsova, D.N. Cherepanov

The effect of impurities of crude olive residue oil on the operational stability of the Candida rugosa lipase immobilized in polyurethane foams Original Research Article
Pages 71-76
A.C. Correia, S. Ferreira-Dias

Approaches for improved identification of mechanisms of enzyme inactivation Original Research Article
Pages 77-82
M. Polakovič, P. Vrábel, V. Báleš

Stability and stabilization of α-1,4-D-glucan phosphorylases Original Research Article
Pages 83-88
R. Grieβler, B. Müller-Fembeck, S. D’Auria, F. La Cara, B. Nidetzky

Oxidation by hydrogen peroxide of D-amino acid oxidase from Rhodotorula gracilis Original Research Article
Pages 89-94
V. Obregón, I. de la Mata, F. Ramón, C. Acebal, M.P. Castillón

Cosolvent effect on the synthesis of ampicillin and cephalexin with penicillin acylase Original Research Article
Pages 95-100
C. Aguirre, J. Baeza, A. Illanes

Conversion and stability studies on enzyme-membrane reactor with lipase immobilized by different methods Original Research Article
Pages 101-106
T. Venyige, E. Csányi, Cs. Sisak

Study of the deactivation process of the glucose oxidase-catalase enzymatic system by means of simulation Original Research Article
Pages 107-112
L.E. Romero, D. Cantero

Stabilization of hydrolases by chemical modification with fatty acids or polyethylene glycol Original Research Article
Pages 115-120
F.J. Plou, M.V. Calvo, M. Ferrer, A. Ballesteros

Modification of the carbohydrate moiety in ribonuclease B and its influence on the protein stability probed by limited proteolysis Original Research Article
Pages 121-126
Ulrich Arnold, Renate Ulbrich-Hofmann

Comparative studies on selective modification of e-amino groups in Upases and phospholipase A2 Original Research Article
Pages 127-134
Bart C. Koops, Arend J. Slotboom, Hubertus M. Verheij

Analysis of the thermal deactivation kinetics of α-chymotrypsin modified by chemoenzymatic glycosylation Original Research Article
Pages 135-140
María Asunción Longo, Didier Combes

Increasing the operational stability of flavoproteins by covalent cof actor binding Original Research Article
Pages 141-146
Willem J.H. van Berkel, Robert H.H. van den Heuvel, Marco W. Fraaije, Colja Laane

Properties of PEG-modified microbial proteases. Activity and stability studies Original Research Article
Pages 147-150
T.M. Fatum, A.Agerlin Olsen, C.C. Fuglsang, D. Otzen

The effect of crosslinking on thermal inactivation of cellulases Original Research Article
Pages 151-156
J. Bilen, U. Bakir

Studies on the stability of acid phosphatase (a. niger) by crosslinking with glutaraldehyde and soil humates Original Research Article
Pages 157-161
N. Ortega, L. Berzal, M.D. Busto, M. Perez-Mateos

Folding and association versus misfolding and aggregation of proteins Original Research Article
Pages 165-182
R. Jaenicke

Activity of monoclonal antibodies in prevention of in vitro aggregation of their antigens Original Research Article
Pages 183-188
Beka Solomon, Tamar Katzav-Gozanski, Rela Koppel, Eilat Hanan-Aharon

Stability of monodeamidated forms of ribonuclease A Original Research Article
Pages 189-196
F. Catanzano, G. Graziano, S. Capasso, G. Barone

Pressure effects on protein oligomeric dissociation Original Research Article
Pages 197-204
C. Balny

Effect of high hydrostatic pressure on enzyme stability Original Research Article
Pages 205-210
V. Athès, D. Combes

Thermodynamic stability of a monomeric derivative of bovine seminal ribonuclease Original Research Article
Pages 211-216
G. Barone, F. Catanzano, G. Graziano, V. Cafaro, G. D’Alessio, A. Di Donato

Thermodynamic stability of ribonuclease P2 from Sulfolobus solfataricus and some mutants Original Research Article
Pages 217-222
G. Graziano, F. Catanzano, P. Fusi, P. Tortora, G. Barone

Stabilization of α-Chymotrypsin by DMSO Original Research Article
Pages 223-228
E. Flaschel, L. Ebmeier

Thermal stabilization by its ligands of NADP+-isocitrate dehydrogenase from the thermophilic cyanobacterium Phormidium laminosum Original Research Article
Pages 229-234
Miguel A. Pardo, María J. Llama, Juan L. Serra

The characterization of protein stability from hydrogen exchange : Maximum entropy analysis of co-solvent effects on exchange rates Original Research Article
Pages 235-240
Badredine Souhail, Isabel M. Plaza del Pino, Jose M. Sanchez-Ruiz

Preferential hydration changes upon protein unfolding in water-cosolvent mixtures Original Research Article
Pages 241-245
Hassan O. Hammou, Isabel M. Plaza del Pino, Jose M. Sanchez-Ruiz

Characterizing protein-cosolvent interactions coupled to protein refolding by kinetic calorimetry Original Research Article
Pages 247-250
Mohammed C. Boulaich, Antonio Parody-Morreale, M.Sánchez-Ruiz José

Characterizing cosolvent effects on protein stability: cold-denaturation of ubiquitin in the presence of guanidine Original Research Article
Pages 251-255
Beatriz Ibarra Molero, Jose M. Sanchez-Ruiz

Acetylcholinesterase-ethanol interactions: inactivation, substrate and ligand exclusion Original Research Article
Pages 257-262
F. Ortéga, D. Garcia, J.-L. Marty

Effect of a mixed stabilizer-denaturant system on the stability of enzyme activity at high temperatures : lysozyme as a model Original Research Article
Pages 263-268
Y. Sangeeta Devi, Usha B. Nair, Rajiv Bhat

A simple folding method for high level production of the hydrophobic disulfide bonded hepatitis B X protein by inclusion body route and its structural analysis Original Research Article
Pages 269-274
I. Marczinovits, J. Molnár, M.Z. Kele, P.T. Szabó, T. Janáky

Rigidity of thermophilic enzymes Original Research Article
Pages 277-294
Angelo Fontana, Vincenzo De Filippis, Patrizia Polverino de Laureto, Elena Scaramella, Marcello Zambonin

Improvement of thermal stability of a diagnostic enzyme, Streptomyces cholesterol oxidase, by random and site-directed mutageneses and a structural interpretation Original Research Article
Pages 295-302
Y. Murooka, Y. Nishiya, M. Toyama, M. Aoike, M. Yamashita, N. Hirayama

Protein engineering for thermostabilisation of proteins: some theoretical rules and application to a β-glucanase Original Research Article
Pages 303-310
E. Querol, J. Pons, J. Cedano, M. Vallmitjana, F. García, C. Bonet, J. Pérez-Pons, A. Planas, A. Mozo-Villarías

Mechanisms of stabilization of the β-glycosidase from the hyperthermophilic archaeon Sulfolobus solfataricus Original Research Article
Pages 311-316
M. Moracci, M. Ciaramella, L.-H. Pearl, B. Cobucci Ponzano, M. Rossi

A strategy for engineering thermostability: the case of cyclodextrin glycosyltransferase Original Research Article
Pages 317-323
Joost C.M. Uitdehaag, Bauke W. Dijkstra

Thermophilic esterases and the amino acid “traffic rule” in the hormone sensitive lipase subfamily Original Research Article
Pages 325-330
Giuseppe Manco, Ferdinando Febbraio, Mosè Rossi

Cloning and stabilization of NAD-dependent formate dehydrogenase from candida boidinii by site-directed mutagenesis Original Research Article
Pages 331-336
H. Slusarczyk, M. Pohl, M.-R. Kula

Usefulness of bacterial thermostable enzymes in clinical chemical analysis Original Research Article
Pages 337-342
K. Tomita, K. Nomura, T. Miura, H. Shibata

Effect of temperature and Ca2+ on the degree of multiple attack exhibited by mesophilic and thermophilic α-amylases Original Research Article
Pages 343-347
B. Kramhøft, B. Svensson

Degradation and denaturation of stable enzymes Original Research Article
Pages 349-352
Dion Thompson, Roberto Fernández-Lafuente, Cesar Mateo, Don A. Cowan, Jose M. Guisan, Roy Daniel

Engineering stability of enzymes in systems with organic solvents Original Research Article
Pages 355-363
Vadim V. Mozhaev

Inactivation of enzymes at the aqueous-organic interface Original Research Article
Pages 365-372
Peter J Halling, Alistair C Ross, George Bell

Exploiting hysteresis for high activity enzymes in organic media Original Research Article
Pages 373-380
Johann Partridge, Peter.J. Halling, Barry D. Moore

Limited proteolysis of proteins by thermolysin in trifluoroethanol Original Research Article
Pages 381-392
Patrizia Polverino de Laureto, Elena Scaramella, Marcello Zambonin, Vincenzo De Filippis, Angelo Fontana

Enzyme inactivation by inert gas bubbling Original Research Article
Pages 393-398
Mylène Caussette, Alain Gaunand, Henri Planche, Brigitte Lindet

Effects of water-miscible solvents on the stability and specificity of cyclodextringlucosyltransferases Original Research Article
Pages 399-404
Anne D. Blackwood, Christopher Bucke

Stabilization of immobilized enzymes against organic solvents: Complete hydrophylization of enzymes environments by solidphase chemistry with poly-functional macromolecules. Original Research Article
Pages 405-410
R. Fernandez-Lafuente, C.M. Rosell, L. Caanan-Haden, L. Rodes, J.M. Guisan

Effect of sorbitol on immobilized a-chymotrypsin thermostability in low-water system Original Research Article
Pages 411-416
T.de Diego, P. Lozano, M.J. Ñíguez, J.L. Iborra.

Stability of immobilized enzyme-polyelectrolyte complex against irreversible inactivation by organic solvents Original Research Article
Pages 417-422
V. Levitsky, P. Lozano, A. Gladilin, J.L. Iborra

Enhancement of invertase activity in organic media for oligosaccharide synthesis Original Research Article
Pages 423-428
S. Bielecki, R.I. Somiari

Effects of crown ethers on the activity of enzymes in peptide formation in organic media Original Research Article
Pages 429-434
Dirk-Jan van Unen, Johan F.J. Engbersen, David N. Reinhoudt

Recovery of the activity of an immobilized lipase after its use in fat transesterification Original Research Article
Pages 435-440
S. Ferreira-Dias, C.S. Duarte, V. Falaschi, S.R. Marques, J.H. Gusmão, M.M.R. da Fonseca

Effects of lipid-borne compounds on the activity and stability of lipases in microaqueous systems for the lipase-catalyzed interesterification Original Research Article
Pages 441-446
X. Xu, C.-E. Høy, J. Adler-Nissen

Stabilisation of lipases for activity in ammoniolysis Original Research Article
Pages 447-452
F. van Rantwijk, A.C. Kock-van Dalen, R.A. Sheldon

Water sorption isotherm as a tool to explore hydration of the microenvironment of biocatalysts Original Research Article
Pages 453-458
J.M. Sánchez-Montero, R.M a de la Casa, J.V. Sinisterra

Thermal stability of free and immobilised Pseudomonas cepacia lipase in aqueous and organic media Original Research Article
Pages 459-464
G. Pencreac’h, Jacques C. Baratti

The stabilization of enzyme in organic solvent at low temperature Original Research Article
Pages 465-470
Hyon Joo Lee, Jin Ryun Kim, Young Je Yoo

A comparative study of thermal inactivation of enzymes in supercritical carbon dioxide Original Research Article
Pages 471-476
A. Gieβauf, T. Gamse, E. Klingsbichel, H. Schwab, R. Marr

Studies on the stability of aminoacylase in some organic solvents Original Research Article
Pages 477-482
L. Boross, J. Kosáry, E. Stefanovits-Bányai, C. Sisak, B. Szajáni

Cutinase activity and enantioselectivity in supercritical fluids Original Research Article
Pages 483-486
Nuno Fontes, M. Conceição Almeida, Sílvia Garcia, Célia Peres, João Grave, M. Raquel Aires-Barros, Cláudio M. Soares, Joaquim M.S. Cabral, Christopher D. Maycock, Susana Barreiros

Effect of pressure on enzyme activity in compressed gases Original Research Article
Pages 487-491
M.Conceição Almeida, Nuno Fontes, Eugénia Nogueiro, Sílvia Garcia, Célia Peres, António Silva, Manuel Carvalho, Susana Barreiros

Activity and structural stability of adsorbed enzymes Original Research Article
Pages 495-504
W. Norde, T. Zoungrana

Covalent immobilization of glucose oxidase on AlPO4 as inorganic support Original Research Article
Pages 505-512
F.M. Bautista, M.C. Bravo, J.M. Campelo, A. García, D. Luna, J.M. Marinas, A.A. Romero.

The effect of site-specific immobilization on the thermal stability of thermolysin-like neutral proteases Original Research Article
Pages 513-516
J. Mansfeld, G. Vriend, B. Van den Burg, G. Venema, V.G.H. Eijsink, R. Ulbrich-Hofmann

Immobilization of hydantoin cleaving enzymes from Arthrobacter aurescens DSM 3747 — Effect of the coupling method on the stability of the L-N-carbamoylase Original Research Article
Pages 517-522
M. Pietzsch, H. Oberreuter, B. Petrovska, K. Ragnitz, C. Syldatk

Thermoinactivation of β-xylosidase immobilized on nylon Original Research Article
Pages 523-528
M.J. Dueñas, P. Estrada

Increase of thermal stability of tannin acyl hydrolase by covalent immobilization through its carbohydrate side chain : application in tea cream solubilisation Original Research Article
Pages 529-534
P. Nicolas, J.L. Sauvageat, S. Reymond, E. Raetz

Stability in the presence of organic solvents of dextransucrase from Leuconostoc mesenteroides NRRL B-512F immobilized in calcium-alginate beads Original Research Article
Pages 535-540
M. Alcalde, F.J. Plou, M.T. Martín, M. Remaud, P. Monsan, A. Ballesteros

Immobilisation and characteristics of glucose oxidase immobilised on convective interaction media (CIM) disks Original Research Article
Pages 541-546
A. Podgornik, M. Vodopivec, H. Podgornik, M. Barut, A. àtrancar

Immobilization of spinach leaf hydroperoxide lyase Original Research Article
Pages 547-552
L.M. Simon, Sz.J. Márczy, M. Kotormán, Sz.Á. Németh, B. Szajáni

A New immobilization system for candida rugosa lipase: characterization and applications Original Research Article
Pages 553-558
K. Carbone, M. Casarci

Soybean lipoxygenases: Purification and stability of the free and immobilized enzymes Original Research Article
Pages 559-564
Anthony Chikere, Boris Galunsky, Volker Kasche

Stabilization of lipase B from Candida antarctica by immobilization on different supports Original Research Article
Pages 565-570
M. Arroyo, J.M. Sánchez-Montero, J.V. Sinisterra

Covalent immobilization of crude and partially-purified Upases onto inorganic supports: stability and hyperactivation Original Research Article
Pages 571-576
A.R. Alcántara, I. Borreguero, M.T. López-Belmonte, J.V. Sinisterra.

Immobilization of alkaline phosphatases on various supports Original Research Article
Pages 577-582
B. Surinenaite, V. Bendikiene, B. Juodka

Stabilization of serine proteases by immobilization Original Research Article
Pages 583-588
V. Bendikiene, B. Juodka

Immobilized cells: Plasmid stability and plasmid transfer Original Research Article
Pages 591-602
J.N. Barbotin, D. Mater, M. Craynest, J.E. Nava Saucedo, N. Truffaut, D. Thomas

Stabilization of immobilized cell systems using a modified metal surface, fructose polymer levan and a high cell concentration Original Research Article
Pages 603-610
M. Bekers, E. Ventina, A. Karsakevich, I. Vina

Plasmid stability in recombinant Saccharomyces cerevisiae expressing the EXG1 gene in free and immobilized cultures Original Research Article
Pages 611-618
A. Guillán, T. Lú Chau, E. Roca, M.J. Núñez, J.M. Lema

Bioreductions by Pyrococcus furiosus at elevated temperatures Original Research Article
Pages 619-624
E. van den Ban, H. Willemen, H. Wassink, H. Haaker, C. Laane

Stability of free and immobilized Mycobacterium sp. cells in aqueous and organic media Original Research Article
Pages 625-630
P. Fernandes, J.M.S. Cabral, H.M. Pinheiro

Operational stability of immobilized C. reinhardtii cells: an approach to its potential as biocatalyst for N-consuming processes Original Research Article
Pages 631-638
I. Garbayo, C. Vílchez

Induction and stability of cholesterol oxidase from cells of a Rhodococcus Original Research Article
Pages 639-644
Joseph Kreit, Pierre Germain

Stability of functionally active fusion proteins during their biosynthesis and isolation from expressing bacterial cells Original Research Article
Pages 645-650
L.M. Vinokurov, A.V. Yakhnin, T.V. Ivashkina, V.N. Ksenzenko, Yu.B. Alakhov

Production of bio-esters by immobilized lipases Original Research Article
Pages 653-656
K. Bélafi-Bakó, L. Gubicza, E. Csányi, M.A Galán, J.A. Moreno

An Aspergillus flavus strain promoting oleic acid esterification in isooctane Original Research Article
Pages 657-660
V. Loscos, G. Cañes, B. Perpiña, M. Torres, N. Sala, R. Canela

Covalent enzyme immobilization in different porous polymer membranes Original Research Article
Pages 661-666
H.-G. Hicke, M. Becker, G. Maisch, M. Ulbricht

Functionalised cross-linked polyvinyl alcohol as new matrix for lipase immobilization Original Research Article
Pages 667-671
E. Cernia, G. Milana, G. Ortaggi, C. Palocci, S. Soro