Microarray Technology and Its Applications

Uwe R. Müller, Dan V. Nicolau9783540229315, 3-540-22931-0

The genomics revolution would not have been possible without the ‘parallelisation’ offered by microarray technology. This technological – and commercial – success has since been emulated by other applications areas, with a tremendous amplification of innovation. This book describes the fundamentals and latest developments in microarray technology, as well as its future directions. It presents detailed overviews of the different techniques of fabricating microarrays, of the chemistries and preparative steps involved, of the different types of microarrays, and of the instrumentation and optical issues involved.

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Table of contents :
Cover……Page 1
Series Title……Page 2
Series Editors……Page 3
Inside Cover……Page 4
Copyright……Page 5
Preface……Page 6
Acknowledgements……Page 9
Contents……Page 11
List of Contributors……Page 17
Part I – General Microarray Technologies……Page 23
1. Array Formats……Page 24
2. Biomolecules and Cells on Surfaces – Fundamental Concepts……Page 44
3. Surfaces and Substrates……Page 66
4. Reagent Jetting Based Deposition Technologies for Array Construction……Page 83
5. Manufacturing of 2-D Arrays by Pin-printing Technologies……Page 93
6. Nanoarrays……Page 109
7. The Use of Microfluidic Techniques in Microarray Applications……Page 139
8. Labels and Detection Methods……Page 166
9. Marker-free Detection on Microarrays……Page 199
Part II – DNA Microarrays……Page 226
10. Analysis of DNA Sequence Variation in the Microarray Format……Page 227
11. High Sensitivity Expression Profiling……Page 244
12. Applications of Matrix-CGH (Array-CGH) for Genomic Research and Clinical Diagnostics……Page 266
13. Analysis of Gene Regulatory Circuits……Page 279
Part III – Protein Microarrays……Page 291
14. Protein, Antibody and Small Molecule Microarrays……Page 292
15. Photoaptamer Arrays for Proteomics Applications……Page 309
16. Biological Membrane Microarrays……Page 321
Part IV – Cell & Tissue Microarrays……Page 333
17. Use of Reporter Systems for Reverse Transfection Cell Arrays……Page 334
18. Whole Cell Microarrays……Page 345
19. Tissue Microarrays for Miniaturized High-Throughput Molecular Profiling of Tumors……Page 354
20. Application of Microarray Technologies for Translational Genomics……Page 370
Index……Page 384