Energetics of Biological Macromolecules

Jo M. Holt, Michael L. Johnson, Gary K. Ackers9780121827830, 0121827836

This volume focuses on the cooperative binding aspects of energetics in biological macromolecules. Methodologies such as NMR, small-angle scattering techniques for analysis, calorimetric analysis, fluorescence quenching, and time resolved FRET measurements are discussed. *Methods for Evaluating Cooperativity in a Dimeric Hemoglobin *Multiple-Binding of Ligands to a Linear Biopolymer *Fluorescence Quenching Methods to Study Protein-Nucleic Acid Interactions *Linked Equilibria in Biotin Repressor Function: Thermodynamic, Structural and Kinetic Analysis