Standardization and Quality Assurance in Fluorescence Measurements I: Techniques

Robert F. Vogt Jr., Gerald E. Marti, Vincent Zenger (auth.), Ute Resch-Genger (eds.)9783540752066, 3540752064

The validation and standardization of fluorescence methods is still in its infancy as compared to other prominent analytical and bioanalytical methods. Appropriate quality assurance standards are however a prerequisite for applications in highly regulated fields such as medical diagnostics, drug development, or food analysis.

For the first time, a team of recognized international experts has documented the present status of quality assurance in fluorescence measurements, and outlines concepts for establishing standards in this field.

This first of two volumes covers basic aspects and various techniques such as steady-state and time-resolved fluorometry, polarization techniques, and fluorescent chemical sensors.

Table of contents :
Front Matter….Pages I-XVI
Front Matter….Pages 1-2
Quantitative Fluorescence Calibration: a Tool for Assessing the Quality of Data Obtained by Fluorescence Measurements….Pages 3-31
Need for and Metrological Approaches Towards Standardization of Fluorescence Measurements from the View of National Metrology Institutes….Pages 33-62
Front Matter….Pages 63-64
Linking Fluorometry to Radiometry with Physical and Chemical Transfer Standards: Instrument Characterization and Traceable Fluorescence Measurements….Pages 65-99
Fluorescence Quantum Yields: Methods of Determination and Standards….Pages 101-145
Long-Wavelength and Near-Infrared Fluorescence: State of the Art, Future Applications, and Standards….Pages 147-162
Surface Fluorescence: the Only Standardized Method of Measuring Luminescence….Pages 163-192
Front Matter….Pages 193-194
Time-Resolved Fluorometry: Typical Methods, Challenges, Applications and Standards….Pages 195-214
Practical Time-Resolved Fluorescence Spectroscopy: Avoiding Artifacts and Using Lifetime Standards….Pages 215-232
Evaluation of Time-Resolved Fluorescence Data: Typical Methods and Problems….Pages 233-258
Time-Resolved Fluorescence: Novel Technical Solutions….Pages 259-275
Front Matter….Pages 277-278
Fluorescence Depolarization Techniques in Materials Science….Pages 279-301
Fluorescence Polarization: Recent Bioanalytical Applications, Pitfalls, and Future Trends….Pages 303-322
Front Matter….Pages 323-324
Classification of Chemical Sensors and Biosensors Based on Fluorescence and Phosphorescence….Pages 325-346
Fibre-Optic and Nanoparticle-Based Fluorescence Sensing Using Indicator Dyes: Pitfalls, Self- Referencing, Application, and Future Trends….Pages 347-372
Intrinsically Referenced Fluorimetric Sensing and Detection Schemes: Methods, Advantages and Applications….Pages 373-414
Total Internal Reflection Fluorescence Sensing – Quality Assurance and Application to Water Analysis….Pages 415-428
Fluorescence Sensing and Imaging Using Pressure-Sensitive Paints and Temperature-Sensitive Paints….Pages 429-461
Front Matter….Pages 463-464
Luminescence Analysis of Actinides: Instrumentation, Applications, Quantification, Future Trends, and Quality Assurance….Pages 465-492
Back Matter….Pages 493-496