Methods of Cell Separation

R.H. Burdon, P.H. van Knippenberg and Paul T Sharpe (Eds.)9780080858876, 9780444809278, 0444809279

Methods of Cell Separation brings to the attention of researchers at all levels the variety of methods available for separating viable populations of cells. Methods are grouped into 3 categories based on the criteria of separation, namely; size or density; non-specific surface properties; and specific surface properties. The principle of each method is described together with general and, where possible, specific protocols for conducting cell separation experiments. A central theme of the book is the separation of populations of blood lymphocytes which is used as an example for each method. Methods of Cell Separation is distinguished by three powerful assets: descriptions of the majority of cell separation methods currently being used; details of the experimental procedures involved in each method; and comparisons of the different methods for separating cell populations with particular reference to blood lymphocytes. An excellent addition to a distinguished series, and extremely useful as a laboratory manual.

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Table of contents :
Content:
Advisory board
Page ii

Edited by
Page iii

Copyright page
Page iv

Abbreviations
Pages v-vi

Chapter 1: Introduction
Pages 1-6

Chapter 2: Methods of cell counting and assaying cell viability
Pages 7-17

Chapter 3: Centrifugation
Pages 18-69

Chapter 4: Unit gravity sedimentation
Pages 70-90

Chapter 5: Centrifugal elutriation
Pages 91-106

Chapter 6: Aqueous two-phase partition
Pages 107-142

Chapter 7: Electrophoresis
Pages 143-158

Chapter 8: Affinity chromatography
Pages 159-186

Chapter 9: Affinity separation on fibres and surfaces
Pages 187-199

Chapter 10: Affinity partition
Pages 200-207

Chapter 11: Flow sorting
Pages 208-220

Chapter 12: Magnetic cell sorting
Pages 221-237

Chapter 13: Separation of cells in different phases of the cell cycle
Pages 238-260

Appendix
Pages 261-263

References Review Article
Pages 264-268

General references for further reading
Page 269

Subject index
Pages 270-272