Pumps, transporters, and ion channels: studies on their structure, function, and cell biology

Francisco Sepulveda, Francisco Bezanilla9780306486593, 0-306-48659-8, 0306486601

Second author, Francisco Benzanilla, is with the Univ. of California, Los Angeles. Presents the proceedings of a symposium on Pumps, Transporters, and Ion Channels: Studies on Their Structure, Function, and Cell Biology, held in Valdivia, Chile in December, 2001.

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Table of contents :
CONTENTS……Page 9
List of Participants……Page 15
2. ION CHANNELS IN BACTERIA……Page 18
3.2 A Cell that is Too Simple……Page 21
3.3 The Solution to the Dilemma……Page 22
3.4 A Thought Experiment on Stopping the Na/K Pump……Page 24
4. CONCLUSIONS……Page 26
5. REFERENCES……Page 27
1. INTRODUCTION……Page 28
3. SINGLE-MOLECULE WATER PERMEABILITY OF AQP0 AND AQP1……Page 30
4. REGULATION OF AQP0 WATER PERMEABILITY……Page 31
5. CONCLUSIONS……Page 34
7. REFERENCES……Page 35
1. INTRODUCTION……Page 37
3. RESULTS AND DISCUSSION……Page 39
5. REFERENCES……Page 45
1. INTRODUCTION……Page 47
2.1 Membrane Preparations……Page 48
3.1.1. Native RyR Channels in Bilayers……Page 49
3.1.2. Release Experiments with Native RyR Channels……Page 50
3.2.1. Oxidized RyR Channels in Bilayers……Page 52
3.2.2. Release Experiments with Oxidized RyR Channels……Page 53
6. REFERENCES……Page 54
1. INTRODUCTION……Page 56
2.1 Membrane Preparation……Page 57
2.2 Channel Recording and Analysis……Page 58
3.1 Brain RyR Channels are Activated by Caffeine and Show the Characteristic Response to Ryanodine……Page 60
3.2 Calcium Dependence of Native and Oxidized RyR-Channels……Page 61
3.3 Activation by ATP……Page 62
3.4 Effect of cADPR……Page 63
4. CONCLUSIONS……Page 64
6. REFERENCES……Page 65
2. THE EXOCYTIC PATHWAY……Page 68
3. POTASSIUM CHANNELS AS SUBSTRATES OF THE EXOCYTIC PATHWAY……Page 70
4. CONCLUDING REMARKS……Page 73
5. REFERENCES……Page 74
1. INTRODUCTION……Page 77
2.1 RNA Synthesis and Preparation of Oocytes……Page 79
2.3 Recording Techniques……Page 80
3. RESULTS……Page 81
5. ACKNOWLEDGMENTS……Page 84
6. REFERENCES……Page 85
2. MOLECULAR SRUCTURE OF VDCC……Page 87
4. CALCIUM CHANNELS AND NEUROTRANSMITTER RELEASE AT THE NEUROMUSCULAR JUNCTION……Page 88
6. REFERENCES……Page 91
1. INTRODUCTION……Page 93
2. PROGRESS TOWARD BK(CA) CHANNEL STRUCTURE……Page 94
3. PROGRESS TOWARD BK(CA) CHANNEL FUNCTION……Page 98
4. REFERENCES……Page 102
1. INTRODUCTION……Page 106
2. SHAKER CHANNEL WORKS WITH S3-S4 SEGMENT DELETED……Page 108
3. RESTORING THE S3-S4 LINKER FIVES CONFUSING RESULTS……Page 110
4. UNDERSTANDING THESE RESULTS……Page 112
6. REFERENCES……Page 114
1. INTRODUCTION……Page 115
2. PORE-SPECIFIC TOXINS FOR POTASSIUM CHANNELS……Page 116
3.1 Binding the Open State……Page 117
4. POSSIBLE ORIGINS FOR THE CLOSED-OPEN STATE DEPENDENCY……Page 118
4.1 Pore Occupancy Modifies Toxin Binding……Page 119
4.2 The Voltage Sensor Interacts with the Toxin Bound to the Pore……Page 120
5. TOXINS BLOCK C-NACTIVATED AND CLOSED CHANNELS EQUALLY……Page 121
5.1 A Plannar Rotation of the Vestibule During Slow Activation……Page 123
8. REFERENCES……Page 124
1. INTRODUCTION……Page 126
2. RESULTS……Page 127
4. REFERENCES……Page 131
1. INTRODUCTION……Page 132
2. METHODS……Page 133
3.1 K[sub(ca)] Currents Track Ambient [Ca[sup(2+)]][sub(AZ)]……Page 134
3.2 K[sub(ca)] Channel Properties and I[sub(Kca)] Calibration……Page 137
3.3 Titration of [Ca[sup(2+)]][sub(AZ)] for Study of Release……Page 140
4. DISCUSSION……Page 141
6. REFERENCES……Page 142
1. INTRODUCTION……Page 144
2.1 Basic Characteristics of SMOCs and K[sub(ca)] Channels underllying SMOCs……Page 145
2.2 SMOCs aare Triggered by Calcium Released from Internal Stores……Page 147
2.3 Calcium Influx Modulates SMOC Activity……Page 149
2.4 Physiological Role of SMOCs in Mudpuppy Parasympathetic Neurons……Page 150
3. CONCLUDING REMARKS……Page 152
5. REFERENCES……Page 153
1. INTRODUCTION……Page 155
1.1 Activation of K[sub(ca)] Channels by Ca[sup(2+)] Sparks……Page 156
2. RESULTS……Page 157
2.1 Out of Tune……Page 158
2.2 Twinkle Twinkle……Page 162
4. ACKNOWLEDGMENTS……Page 163
5. REFERENCES……Page 165
2. OVERVIEW OF ClC CHANNELS WITH EMPHASIS ON ClC-2……Page 167
3. RESULTS……Page 169
5. REFERENCES……Page 173
EDITORS’ NOTE……Page 175