Plant Metabolomics

J. Kopka (auth.), Professor Dr. Kazuki Saito, Professor Dr. Richard A. Dixon, Professor Dr. Lothar Willmitzer (eds.)3-540-29781-2, 978-3-540-29781-9

Metabolomics – which deals with all metabolites of an organism – is a rapidly-emerging sector of post-genome research fields. It plays significant roles in a variety of fields from medicine to agriculture and holds a fundamental position in functional genomics studies and their application in plant biotechnology.

This volume comprehensively covers plant metabolomics for the first time. The chapters offer cutting-edge information on analytical technology, bioinformatics and applications. They were all written by leading researchers who have been directly involved in plant metabolomics research throughout the world. Up-to-date information and future developments are described, thereby producing a volume which is a landmark of plant metabolomics research and a beneficial guideline to graduate students and researchers in academia, industry, and technology transfer organizations in all plant science fields.

Table of contents :
Front Matter….Pages 1-1
Gas Chromatography Mass Spectrometry….Pages 3-20
Current Status and Forward Looking Thoughts on LC/MS Metabolomics….Pages 21-32
Plant Metabolomics Strategies Based upon Quadrupole Time of Flight Mass Spectrometry (QTOF-MS)….Pages 33-48
Capillary HPLC….Pages 49-63
Capillary HPLC Coupled to Electrospray Ionization Quadrupole Time-of-flight Mass Spectrometry….Pages 65-79
NMR Spectroscopy in Plant Metabolomics….Pages 81-91
Hetero-nuclear NMR-based Metabolomics….Pages 93-101
Front Matter….Pages 103-103
Bioinformatics Approaches to Integrate Metabolomics and Other Systems Biology Data….Pages 105-115
Chemometrics in Metabolomics — An Introduction….Pages 117-128
Map Editor for the Atomic Reconstruction of Metabolism (ARM)….Pages 129-139
AraCyc: Overview of an Arabidopsis Metabolism Database and its Applications for Plant Research….Pages 141-154
KaPPA-View: A Tool for Integrating Transcriptomic and Metabolomic Data on Plant Metabolic Pathway Maps….Pages 155-163
KNApSAcK: A Comprehensive Species-Metabolite Relationship Database….Pages 165-181
Front Matter….Pages 183-183
Systems Biology: A Renaissance of the Top-down Approach for Plant Analysis….Pages 185-198
Systems-based Analysis of Plant Metabolism by Integration of Metabolomics with Transcriptomics….Pages 199-209
Targeted Profiling of Fatty Acids and Related Metabolites….Pages 211-228
Metabolic Profiling and Quantification of Carotenoids and Related Isoprenoids in Crop Plants….Pages 229-242
Metabolomics and Gene Identification in Plant Natural Product Pathways….Pages 243-259
Metabolomic Analysis of Catharanthus roseus Using NMR and Principal Component Analysis….Pages 261-276
Metabolomics of Plant Secondary Compounds: Profiling of Catharanthus Cell Cultures….Pages 277-289
Front Matter….Pages 183-183
The Taxus Metabolome and the Elucidation of the Taxol® Biosynthetic Pathway in Cell Suspension Cultures….Pages 291-309
The Use of Non-targeted Metabolomics in Plant Science….Pages 311-325
Plant Metabolite Profiling for Industrial Applications….Pages 327-339