Sequencing of Proteins and Peptides

G. Allen (Eds.)0444802754, 9780444802750

Although there is a continuing high level of activity in direct studies on protein primary structures, the requirement for partial sequences has provided a major impetus for the development and dissemination of highly sensitive techniques. While retaining the still applicable formidable basis of the original volume, this Revised Edition reflects the shift towards the use of more sophisticated automated high-performance chromatographic techniques. Sequencing of Proteins and Peptides provides you with the most up-to-date information selected to be the most appropriate for laboratory or reference use since, as in the first edition, it is impossible to cover the field of protein sequence determination fully. New information covers: •FABMS •protein recovery methods •Amino acid analysis •Tandem, plasma description, FAB, and Thermospray mass spectrometry. As in the First edition, Appendixes give information on manufacturers and supplies, purification, and safety in the lab.

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Table of contents :
Content:
Advisory board
Page ii

Edited by
Page iii

Copyright page
Page iv

Preface
Pages v-vii

List of abbreviations
Pages ix-x

Chapter 1 Introduction
Pages 1-15

Chapter 2 Preliminary characterization of the protein
Pages 17-42

Chapter 3 Specific cleavage of the protein
Pages 43-71

Chapter 4 Separation and purification of peptides
Pages 73-133

Chapter 5 Methods for the detection of peptides
Pages 135-160

Chapter 6 Methods for the detection of peptides
Pages 161-236

Chapter 7 Structures introduced by post-translational modification
Pages 237-256

Chapter 8 Deduction of the total primary structure of a protein from the sequences of constituent peptides
Pages 257-262

Chapter 9 Integral membrane proteins
Pages 263-272

Chapter 10 Applications of partial sequence analysis
Pages 273-282

Appendix I Manufacturers and suppliers of equipment and reagents for. protein sequence analysis
Pages 283-291

Appendix II Purification of solvents and reagents
Pages 291-294

Appendix III Safety in the protein chemistry laboratory
Pages 294-295

Appendix IV Notes on work with very small amounts of proteins and peptide
Pages 295-297

References
Pages 299-318

Subject index
Pages 319-327