Functional Nucleic Acids for Analytical Applications

Yi Lu, Yingfu Li (auth.), Li Yingfu, Lu Yi (eds.)0387737103, 978-0-387-73710-2, 978-0-387-73711-9

Both DNA and RNA can serve purposes beyond simple genetic storage and transfer – for example, they can also be employed as enzymes (for catalysis) and receptors (for ligand binding). Increasingly, researchers are making interesting use of these molecules, now collectively called functional nucleic acids. This book, containing chapters written by leading experts in relevant fields, provides timely accounts of diverse analytical applications that take advantage of the functional nucleic acids. The book is written for academic, clinical and industrial scientists and graduate students who may consider exploring functional nucleic acids in their research or practice.

Table of contents :
Front Matter….Pages i-viii
Introductory Remarks….Pages 3-9
Natural Functional Nucleic Acids: Ribozymes and Riboswitches….Pages 11-46
Artificial Functional Nucleic Acids: Aptamers, Ribozymes, and Deoxyribozymes Identified by In Vitro Selection….Pages 47-108
Fluorescent Aptamer Sensors….Pages 111-130
Fluorescent Ribozyme and Deoxyribozyme Sensors….Pages 131-153
Colorimetric and Fluorescent Biosensors Based on Directed Assembly of Nanomaterials with Functional DNA….Pages 155-178
Electrochemical Approaches to Aptamer-Based Sensing….Pages 179-197
Amplified DNA Biosensors….Pages 199-252
Aptamers in Affinity Separations:Capillary Electrophoresis….Pages 255-270
Aptamers in Affinity Separations: Stationary Separation….Pages 271-286
Aptamer Microarrays….Pages 287-308
The Use of Functional Nucleic Acids in Solid-Phase Fluorimetric Assays….Pages 309-342
Functional Nucleic Acid Sensors as Screening Tools….Pages 343-354
Nucleic Acids for Computation….Pages 355-375
DNAzymes in DNA Nanomachines and DNA Analysis….Pages 377-388
Back Matter….Pages 389-395