Colorectal Cancer: Methods and Protocols

Steven M. Powell9780896037670, 9781592590841, 0896037673

Instructs investigators in key genetic, cellular, and molecular biological methods of analyzing colorectal tumors. Techniques and assays are described in detail to allow researchers to experiment on colon tumors. Most chapters are written by scientists who pioneered the methods presented. Coverage includes gross microdissection of colon tumors, establishment of cell lines and xenografted tumors, chromosomal analyses such as FISH assays, mutational analyses of colon tumors and blood samples, gene expression analyses, and microsatellite instability testing. Includes a few color images. For researchers at various levels, from graduate students to professionals. Powell is affiliated with the University of Virginia Health System.