Molecular Biology Problem Solver

Alan S. Gerstein9780471379720, 0471379727

Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as: * Have you selected the most appropriate research strategy? * Have you identified the issues critical to your successful application of a technique? * Are you familiar with the limitations of a given technique? * When should common procedural rules of thumb not be applied? * What strategies could you apply to resolve a problem? A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book’s expert contributors include: * Working safely with biological samples and radioactive materials * DNA and RNA purification * PCR * Protein and nucleid acid hybridization * Prokaryotic and eukaryotic expression systems * Properly using and maintaining laboratory equipment