Cell Separation Methods and Applications

Diether Recktenwald9780824798642, 0-8247-9864-3

This unique, single-source volume offers complete coverage and assessment of cell separation technologies for analytical and preparative isolations of biological cell populations–demonstrating how to select and devise optimal sorting strategies for applications in biochemistry, immunology, cell and molecular biology, and clinical research. Cell Separation Methods and Applications discusses the basic technical principles underlying, as well as the latest developments in lytic removal of cell subsets density gradient separations biotin avidin immunochromatography complement-based cell lysis flow cytometry-based fluorescence-activated cell sorting centrifugal elutriation polystyrene immunoaffinity devices high-density particles immunomagnetic cell sorting magnetophoresis In addition, the book features possibilities and imitations of cell purification in clinical cellular treatment for transplantation, AIDS, and gene therapy combinations of separation techniques in molecular and cell biology and genetics, as well as clinical isolation of stem and progenitor cells for cancer treatment appendices furnishing cell properties and a CD antigen table updated with results from a leukocyte antigen workshop held recently in Osaka, Japan examples supplemented with performance data and more! Enhanced with useful references, tables, equations, drawings, photographs, and micrographs, this outstanding resource is essential reading for biochemical engineers, biochemists, cell and molecular biologists, immunologists, clinicians involved in cell transplantation, designers of cell separation systems, industrial microbiologists, oncologists, and graduate-level students in these disciplines.