PCR Mutation Detection Protocols

Andrea M. Guilliatt (auth.), Bimal D. M. Theophilus, Ralph Rapley (eds.)9780585403304, 9780896036178, 0896036170

Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.

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Table of contents :
Front Matter….Pages i-x
Back Matter….Pages 1-12
….Pages 13-15