Polymeric gene delivery: principles and applications

Mansoor M. Amiji084931934X, 9780849319341, 9780203500477

To treat disease or correct genetic disorders using gene therapy, the most suitable vehicle must be able to deliver genes to the appropriate tissues and cells in the body in a specific as well as safe and effective manner. While viruses are the most popular vehicles to date, their disadvantages include toxicity, limited size of genes they can carry, and limited scale of industrial production. Polymeric Gene Delivery: Principles and Applications is the first comprehensive book to specifically address polymeric gene delivery systems. Uniting the expertise of international academic and industrial scientists who are working in the area of polymeric vectors for gene delivery, it is written by prominent researchers directly involved in this field. The book is divided into five sections that deal with challenges and opportunities in gene delivery and the efficient delivery of genes into somatic cells using polymeric vectors. The authors discuss using biodegradable polymers, condensing and non-condensing polymeric systems, microspheres and nanospheres, and designing specialized delivery systems based on targeting strategies. Polymeric Gene Delivery: Principles and Applications accentuates the versatility of polymeric delivery systems, including the potential for biocompatibility, the ability to design their formulation and geometry for a specific purpose, and the ease of modification to the surface of polymeric carriers. This book is an up-to-date guide for researchers in the field and those interested in entering this dynamic field.

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Table of contents :
Book Cover……Page 1
Half-Title……Page 2
Title……Page 4
Copyright……Page 5
Dedication……Page 6
Preface……Page 8
Contributors……Page 11
Table of contents……Page 19
1 Introduction……Page 22
Part I Gene delvery: Challenges and opportunities……Page 24
2 Tissue-and specific targeting for the delivery of genetic information……Page 25
3 Biological barriers to gene transfer……Page 52
4 Cellular uptake and trafficking……Page 67
5 Pharmacokinetics of polymer-plasmid DNA complex……Page 88
Part II Condesing polymeric systems……Page 104
A. Non-degeradable polymers……Page 105
6 Poly(L-Lysine) and copolymers for gene delivery……Page 106
7 Gene delivery using polyethylenimine abd copolymers……Page 125
8 Poly((2-(dimethylamino)ethyl methacrylate)-Based polymers for the delivery of genes in vitro and in vivo……Page 137
9 Cationic dendrimers as gene transfection vectors: Dendri-poly(amidoamines ) and Dendri-poly(propylenimines)……Page 173
10 Poly(ethylene glycol)-conjugated cationic dendrimers……Page 202
11 Water Soluble Lipopolymers for Gene Delivery……Page 221
12 Cyclodextrin-Containing Polymers for Gene Delivery……Page 236
B. Biodegradable Polymers……Page 266
13 Gene Delivery Using Polyimidazoles and Related Polymers……Page 267
14 Degradable Poly(β-amino ester)s for Gene Delivery……Page 283
15 Cationic Polyesters as Biodegradable Polymeric Gene Delivery Carriers……Page 302
16 Poly(amidoamine)s for Gene Delivery……Page 317
17 Cationic Polysaccharides for Gene Delivery……Page 343
18 Gene Delivery Using Chitosan and Chitosan Derivatives……Page 369
PART III Non-Condensing Polymeric Systems……Page 381
19 Pluronic® Block Copolymers for Nonviral Gene Delivery……Page 382
20 Use of Poly(N-vinyl pyrrolidone) with Noncondensed Plasmid DMA Formulations for Gene Therapy and Vaccines……Page 401
21 Use of HPMA Copolymers in Gene Delivery……Page 427
PART IV Polymeric Nanospheres and Microspheres……Page 438
A. Polymeric Nanospheres……Page 439
22 Biodegradable Nanoparticles as a Gene Expression Vector……Page 440
23 Nanoparticles Made of Poly(lactic acid) and Poly(ethylene oxide) as Carriers of Plasmid DMA……Page 453
24 Poly(alkylcyanoacrylate) Nanoparticles for Nucleic Acid Delivery……Page 476
25 Layer-by-Layer Nanoengineering with Polyelectrolytes for Delivery of Bioactive Materials……Page 490
26 Ex Vivo and In Vivo Adenovirus-Mediated Gene Delivery into Refractory Cells via Nanoparticle Hydrogel Formulation……Page 513
27 Protein Nanoparticles for Gene Delivery……Page 527
B. Polymeric Microspheres……Page 551
28 Gene Delivery Using Poly(lactide-co-glycolide) Microspheres……Page 552
29 Polyanhydride Microspheres for Gene Delivery……Page 573
30 Microspheres Formulated from Native Hyaluronan for Applications in Gene Therapy……Page 582
PART V Specialized Delivery Systems……Page 598
31 Genetically Engineered Protein-Based Polymers: Potential in Gene Delivery……Page 599
32 Glycopolymer Tools for Studying Targeted Nonviral Gene Delivery……Page 624
33 Targeted Gene Delivery via the Folate Receptor……Page 639
34 Transferrin Receptor-Targeted Gene Delivery Systems……Page 654
35 Gene Delivery to the Lungs……Page 678
36 Cutaneous Gene Delivery……Page 697
37 Enhancement of Wound Repair by Sustained Gene Transfer via Hyaluronan Matrices……Page 717
38 Gene Delivery from Tissue Engineering Matrices……Page 745
39 Gene Therapy Stents for In-Stent Restenosis……Page 760
40 Gene Delivery Using BioMEMS……Page 803
Index……Page 818