Nuclear Receptors

David W. Russell9780121822675, 0121822672

Nuclear Receptors are inducible transcription factors that mediate complex effects on development, differentiation and homeostasis. They regulate the transcription of their target genes through binding to DNA sequences.

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Table of contents :
29.pdf……Page 0
Preface……Page 1
Table of Contents……Page 3
Contributors to Volume 353……Page 6
Introduction……Page 12
General Considerations……Page 14
Solid-Phase Extraction……Page 16
Reversed-Phase HPLC……Page 19
How Much Material is Required for Chemical Characterization?……Page 22
Activity-Guided Fractionation……Page 23
Transfection Protocol……Page 25
Luciferase and beta-Galactosidase Assays……Page 27
Structure Determination by Mass Spectrometry……Page 29
Exact Mass Measurement by ES-MS……Page 30
Electron-Impact Mass Spectrometry……Page 31
Concluding Remarks……Page 32
Biological Significance and Available Methods……Page 34
Content of Oxysterols in Tissues……Page 35
Methodology. Internal Standards……Page 37
Synthesis of [2H6]4beta-Hydroxycholesterol……Page 38
Synthesis of [2H6]7beta-Hydroxycholesterol and [2H6]7-Oxocholesterol……Page 39
Sample Preparation……Page 40
Determination of Oxysterols in Human Plasma