Retention and Selectivity in Liquid Chromatography: Prediction, Standardisation and Phase Comparisons

Roger M. Smith (Eds.)0444815392, 9780444815392

This book brings together a number of studies which examine the ways in which the retention and selectivity of separations in high-performance liquid chromatography are dependent on the chemical structure of the analytes and the properties of the stationary and mobile phases. Although previous authors have described the optimisation of separations by alteration of the mobile phase, little emphasis has previously been reported of the influence of the structure and properties of the analyte.
The initial chapters describe methods based on retention index group increments and log P increments for the prediction of the retention of analytes and the ways in which these factors are influenced by mobile phases and intramolecular interactions. The values of a wide range of group increments in different eluents are tabulated.
Different scales of retention indices in liquid chromatography are described for the comparison of separations, the identification of analytes and the comparison of stationary phases. Applications of these methods in the pharmaceutical, toxicology, forensic, metabolism, environmental, food and other fields are reviewed. The effects of different mobile phases on the selectivity of the retention indices are reported. A compilation of sources of reported retention index values are given.
Methods for the comparison of stationary phases based on the interactions of different analytes are covered, including lipophilic and polar indices, shape selectivity comparisons, their application to novel stationary phases, and chemometric methods for column comparisons.

---

Table of contents :
Content:
Edited by
Page III

Copyright page
Page IV

Preface
Pages XIII-XIV
Roger M. Smith

List of Contributors
Page XV

Chapter 1 Retention prediction based on molecular structure Original Research Article
Pages 1-46
Roger M. Smith

Chapter 2 Retention prediction of pharmaceutical compounds Original Research Article
Pages 47-92
Klára Valkó

Chapter 3 Retention index scales used in high-performance liquid chromatography Original Research Article
Pages 93-144
Roger M. Smith

Chapter 4 Application of retention indices for identification in high-performance liquid chromatography Original Research Article
Pages 145-169
Roger M. Smith

Chapter 5 Application of nitroalkanes and secondary retention index standards for the identification of drugs Original Research Article
Pages 171-207
Maciej Bogusz

Chapter 6 Identification using retention indices in gradient HPLC Original Research Article
Pages 209-233
P. Kuronen

Chapter 7 Characterization of retention and selectivity in reversed-phase LC using interaction indices Original Research Article
Pages 235-267
P. Jandera

Chapter 8 Lipophilic and polar indices Original Research Article
Pages 269-295
P. Jandera

Chapter 9 Solvent selectivity Original Research Article
Pages 297-336
S.D. West

Chapter 10 Retention and selectivity for polycyclic aromatic hydrocarbons in reversed-phase liquid chromatography Original Research Article
Pages 337-369
Lane C. Sander, Stephen A. Wise

Chapter 11 Comparison of novel stationary phases Original Research Article
Pages 371-401
Joseph J. Pesek, Eric J. Williamsen

Chapter 12 Multivariate characterization of RP-HPLC stationary phases Original Research Article
Pages 403-449
Annabel Bolck, Age K. Smilde

Subject Index
Pages 451-457