In Vitro Mutagenesis Protocols

Priya Handa, Swapna Thanedar, Umesh Varshney (auth.), Jeff Braman (eds.)9780585403021, 9780896039100, 0896039102

Hands-on researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. The contributors focus on improvements to conventional site-directed mutagenesis, including a chapter on chemical site-directed mutagenesis, PCR-based mutagenesis and the modifications that allow high throughput mutagenesis experiments, and mutagenesis based on gene disruption (both in vitro- and in situ-based). Additional methods are provided for in vitro gene evolution; for gene disruption based on recombination, transposon, and casette mutagenesis; and for facilitating the introduction of multiple mutations. Time-tested and highly practical, the protocols in In Vitro Mutagenesis Protocols, 2nd Edition offer today’s molecular biologists reliable and powerful techniques with which to illuminate the proteome.

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Table of contents :
Front Matter….Pages i-xvi
Back Matter….Pages 1-6
….Pages 7-17