Plasmids: A Practical Approach

K. G. Hardy9780199634446, 0199634440

This completely updated and revised second edition of Plasmids: A Practical Approach provides the researcher with detailed protocols for studying bacterial plasmids and for using plasmids and phagemids as vectors. With many new sections and protocols, it is the most up-to-date reference describing the techniques for the study of plasmid replication and maintenance, the fundamental methods for purifying plasmids and introducing them into cells, and the protocols for the mutagenesis and analysis of plasmid-encoded products. The book features further techniques for the study of plasmids and the use of plasmid vectors in such important groups of bacteria as animal and plant pathogens harboring virulence plasmids, Streptomyces, and lactococci. Plus, the book describes the use of plasmid composite vectors for expression of cDNA cloning, and much more. It is a invaluable reference for molecular biologists, microbiologists, and biotechnology researchers. It includes detailed protocols for studying bacterial plasmids and for using both plasmids and phagemids as vectors, techniques for studying fundamental aspects of plasmids, such as replication, are included, as well as methods for using plasmid vectors in important groups of bacteria. Also described are protocols for using phagemids, which are extremely useful tools for genetic engineering.

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Table of contents :
covering……Page 1
The practical approach series……Page 2
Preface……Page 4
Contents……Page 5
Contributors……Page 8
Abbreviations……Page 9
1-Plasmid replication and maintenace……Page 10
2-Plasmids from Gram-positive bacteria……Page 29
3-Lactoccal plasmid vectors……Page 42
4-Virulence plasmids……Page 57
5-Plasmids in Agrobacterium tumefaciens and Rhodococcus fascians……Page 69
6-Streptomyces plasmid vectors……Page 83
7-Use of Lambda-plasmid composite vectors for expression cDNA cloning……Page 98
8-Phagemids and other hybrid vectors……Page 108
9-Extrachromosomal cloning vectors of Saccharomyces cerevisiae……Page 122
A1-Addresses of suppliers……Page 131
Index……Page 132
Order other titles of interest today……Page 136