Guide to Protein Purification

John N. Abelson0121820831, 9780121820831

Guide to Protein Purification, designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source.Key Features* Detailed procedures newly written for this volume* Extensive practical information* Rationale and strategies for protein and enzyme purification* Personal perspectives on enzyme purification by eminent researchersAmong the Topics Covered* General methods for handling proteins and enzymes* Extraction, subcellular fractionation, and solubilization procedures* Comprehensive purification techniques* Specialized purification procedures* Protein characterization* Immunological procedures* Computer analysis of protein structure