Transgenic Plants: Methods and Protocols

Luis Herrera-Estrella, June Simpson, Miguel Martínez-Trujillo (auth.), Leandro Peña (eds.)9781588292636, 9781592598274, 1588292630

A collection of readily reproducible techniques for the genetic transformation and regeneration of plants. The authors cover the most commonly used transformation systems for the regeneration of whole transgenic plants. Among the techniques discussed for the detection of transgenes are quantitative real-time PCR, RT-PCR, in situ hybridization, FISH, and TAIL-PCR. Risk assessment methods offer the opportunity to study Agrobacterium persistence in plant tissues and to investigate the possibility of transgene dispersal. The protocols follow the successful Methods in Molecular Biologyв„ў series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

---

Table of contents :
Front Matter….Pages i-xiv
Front Matter….Pages 1-1
Front Matter….Pages 3-31
Front Matter….Pages 33-33
Front Matter….Pages 35-46
Front Matter….Pages 47-60
Front Matter….Pages 61-78
Front Matter….Pages 79-90
Front Matter….Pages 91-101
Back Matter….Pages 103-109
….Pages 111-137