Primrose – Principles of Gene Manipulation

Table of contents :
1 – Gene manupulation: an all embracing techinque……Page 2
2 – Basic techniques……Page 9
3 – Cutting and joining DNA molecules……Page 26
4 – Basic biology of plasmids and phage vectors……Page 44
5 – Cosmids, phasmids and other advanced vectors……Page 65
6 – Cloning strategies……Page 86
7 – Sequencing and mutagenesis……Page 120
8 – Cloning in bacteria other than E. coli……Page 140
9 – Cloning in S. cervisiae and other fungi……Page 157
10 – Gene transfer to animal cells……Page 174
11 – Gene manipulation of animals……Page 203
12 – Gene transfer to plants……Page 222
13 – Advances in transgenic technology……Page 248
14 – Applications of recombinant DNA technology……Page 275