Evaluation of Analytical Methods in Biological Systems Analysis of Biogenic Amine part A

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Table of contents :
Front Cover……Page 1
Analysis of Biogenic Amines……Page 4
Copyright Page……Page 5
Contents……Page 6
Contributors……Page 14
Preface……Page 16
1.2. Catecholamines and their O-methylated metabolites……Page 18
1.3. 5-Hydroxytryptamine……Page 20
1.4. Trace amines……Page 21
1.5. Histamine……Page 24
1.6. Purpose of the book……Page 25
References……Page 26
2.1. General problems in bioassay……Page 32
2.2. Design of experiments……Page 34
2.3. Choice of tissues……Page 40
2.4. Methods of assay……Page 45
2.5. Conclusions……Page 52
References……Page 53
3.1. Introduction……Page 54
3.2. TLC as applied to the biogenic amines……Page 58
References……Page 76
4.1. Introduction……Page 80
4.2. Fluorescence techniques for detection of amines……Page 83
4.3. Fluorescence techniques for quantitation of amines……Page 85
4.4. Comparison of fluorescence techniques and other analytical methods……Page 94
References……Page 95
5.1. Introduction……Page 100
5.2. Chemical background to the formaldehyde and glyoxylic acid method……Page 102
5.3. Practical aspects of the histochemical detection of the monoamines……Page 112
5.4. Evaluation of the formaldehyde and glyoxylic acid techniques……Page 119
References……Page 123
6.1. Introduction……Page 126
6.2. Analysis of specific amines……Page 130
6.3. Advantages and limitations of GC compared to other analytical methods……Page 141
References……Page 142
7.1. Introduction……Page 146
7.2. Procedure……Page 149
7.3. Evaluation of high resolution mass spectrometry vs. other analytical techniques……Page 152
7.4. Summary and promising developments……Page 160
Acknowledgements……Page 162
References……Page 163
8.1. Introduction……Page 168
8.2. Experimental approaches……Page 175
8.3. Special techniques……Page 185
8.4. Selected applications……Page 190
8.5. Comparison with other analytical procedures……Page 193
8.6. Summary……Page 195
References……Page 196
9.2. The catecholamines……Page 200
9.3. Precursors and metabolites of the catecholamines……Page 207
9.4. 5-Hydroxytryptamine (Serotonin; 5-HT)……Page 209
9.5. Histamine……Page 211
9.6. The trace amines……Page 213
9.7. Conclusions……Page 216
References……Page 217
10.1. Introduction……Page 220
10.2. Principles of HPLC separation and detection of biogenic amines……Page 221
10.3. Determination of biogenic amines in biological samples……Page 243
10.4. Summary……Page 249
References……Page 250
11.1. Voltammetry……Page 254
11.2. Liquid chromatography with electrochemical detection……Page 264
11.3. In vivo electrochemistry……Page 268
References……Page 280
12.1. Introduction……Page 284
12.2. Competitive binding assays……Page 285
12.3. Assay procedures……Page 291
12.4. Sensitivity……Page 295
12.5. Specificity……Page 296
12.6. Applications……Page 297
12.7. Summary……Page 298
References……Page 299
13.1. Introduction……Page 302
13.2. Radioimmunoassay of phenalkylamines……Page 304
13.3. Conclusion……Page 315
References……Page 317
Subject Index……Page 319