Handbook of Molecular-Genetic Techniques for Brain and Behavior Research

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Edition: 1st ed

Series: Techniques in the behavioral and neural sciences 13

ISBN: 0444502394, 9780444502391

Size: 19 MB (19450084 bytes)

Pages: 3-965/297

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W.E. Crusio and R.T. Gerlai (Eds.)0444502394, 9780444502391

The book gives a broad overview of recombinant DNA techniques for the behavioral neuroscientist, with illustrative examples of applications. Species covered include rodents (mainly mice), Drosophila melanogaster, Caenorhabditis elegans and Danio rerio. Experimental techniques required to characterize the behavioral phenotypes of mutant animals is provided. Several aspects of novel molecular-genetic techniques are overviewed and possible research strategies are explained. The sections of the book start with general descriptions of techniques followed by illustrative examples. It is divided into six sections. Section 1, bioinformatics and genomics research. Section 2, top-down strategies, where the researcher starts with the phenotype and then analyzes the associated genes; bottom-up strategies, where the physiological chain leading to a phenotype is analyzed starting from the gene product. Section 3, transgenic approaches in rodents including overexpressing foreign genes and gene-targeting; systemic manipulation approaches directly targeting the central nervous system and methods used with invertebrates. Section 4, methods used to evaluate relevant behavioral phenotypes, including learning and aggression. Section 5, examples on molecular brain research in man. Section 6, ethical aspects of research in this field.

Table of contents :
Content:
Preface. Behavioral neurogenetics: the new synthesis
Pages vii-x
Wim E. Crusio, Robert T. Gerlai

Acknowledgements
Pages xi-xii

List of contributors
Pages xiii-xix

Chapter 1.1 The multi-dimensional database requirements of brain information in the era of rapid gene identification Original Research Article
Pages 3-19
Floyd E. Bloom, Warren G. Young

Chapter 1.2 Bioinformatics and neuroscience in the post-genomic era Original Research Article
Pages 20-30
Bruno W.S. Sobral, Michael M. Harpold

Chapter 1.3 PsycheMap — An online database of psychiatric genetics linkage, association, and genome mapping projects Original Research Article
Pages 31-39
Nigel M. Williams, Iain Fenton, Michael J. Owen

Chapter 1.4 Experimental design and statistical inference Original Research Article
Pages 40-57
Douglas Wahlsten

Chapter 2.1.1 Mapping single locus mutations in mice: towards gene identification of neurological traits Original Research Article
Pages 61-81
Wayne N. Frankel, Benjamin A. Taylor

Chapter 2.1.2 Experimental strategies for quantitative trait loci (QTL) analysis in laboratory animals Original Research Article
Pages 82-99
David A. Blizard, Ariel Darvasi

Chapter 2.1.3 Linkage strategies for mapping genes for complex traits in man Original Research Article
Pages 100-112
Laura Almasy, John Blangero

Chapter 2.1.4 Genetic association studies in behavioral neuroscience Original Research Article
Pages 113-121
Philip Gorwood

Chapter 2.1.5 DNA pooling in allelic association studies Original Research Article
Pages 122-131
Nigel M. Williams, Michael J. Owen

Chapter 2.1.6 Research strategies for the analysis of neurological mutants of the mouse Original Research Article
Pages 132-146
Constantino Sotelo, Jean Mariani

Chapter 2.1.7 Genetic dissection of mouse behavior using induced mutagenesis Original Research Article
Pages 147-165
Lawrence H. Pinto, Joseph S. Takahashi

Chapter 2.1.8 Mutagenesis in zebra fish: studying the brain dopamine systems Original Research Article
Pages 166-176
Su Guo, Wolfgang Driever, Arnon Rosenthal

Chapter 2.1.9 Behavioral and electrophysiological screens for isolating zebra fish mutants with visual system defects Original Research Article
Pages 177-186
John E. Dowling

Chapter 2.2.1 Subtractive cDNA hybridization and brain: then, now and tomorrow Original Research Article
Pages 189-199
Joseph B. Watson

Chapter 2.2.2 Applying differential display to brain research Original Research Article
Pages 200-211
Iaudio V. Mello, Erich D. Jarvis

Chapter 2.2.3 Brain region-specific genes: the hippocampus Original Research Article
Pages 212-224
Benjamin S. Pickard, Ben J. Davies, Ken A. Rose, Genevieve Stapleton, Muriel Steel, Richard Lathe

Chapter 2.2.4 Application of real-time RT-PCR for quantification of gene expression Original Research Article
Pages 225-236
Jane Winer, Natasha Shinsky, Robert Gerlai, P. Mickey Williams

Chapter 2.2.5 Analyzing genomic DNA discordance between monozygotic twins Original Research Article
Pages 237-256
J. Bouchard, C. Foulon, N. Storm, G.H. Nguyen, C.L. Smith

Chapter 3.1.1 Embryonic stem cells and gene targeting Original Research Article
Pages 259-271
Anthony Wynshaw-Boris, Lisa Garrett, Amy Chen, Carrolee Barlow

Chapter 3.1.2 Generation of transgenic mice by pronuclear DNA injection Original Research Article
Pages 272-281
Anthony Wynshaw-Boris, Lisa Garrett, Amy Chen, Carrolee Barlow

Chapter 3.1.3 Brain region-specific and temporally restricted gene knockout using the Cre recombinase system Original Research Article
Pages 282-290
Joe Z. Tsien

Chapter 3.1.4 Regulated temporal and spatial expression of mutants of CaMKII and calcineurin with the tetracycline-controlled transactivator (tTA) and reverse tTA (rtTA) systems Original Research Article
Pages 291-304
Isabelle M. Mansuy, Mark Mayford, Eric R. Kandel

Chapter 3.1.5 The use of targeted point mutants in the study of learning and memory Original Research Article
Pages 305-314
Karl Peter Giese

Chapter 3.1.6 Genetic dissection of a postsynaptic multiprotein complex controlling synaptic plasticity and learning in the mouse Original Research Article
Pages 315-328
Seth G.N. Grant

Chapter 3.1.7 Molecular genetic analysis of glutamate receptor function in long-term potentiation in the mouse hippocampus Original Research Article
Pages 329-340
Zhengping Jia, You Ming Lu, Nadia Agopyan, John Roder

Chapter 3.1.8 Targeting aggression in mice Original Research Article
Pages 341-351
Randy J. Nelson, Lance J. Kriegsfeld

Chapter 3.1.9 Behavioral analysis of Dvl1-deficient mice reveals a role for the Dvl1 gene in social behaviors and sensorimotor gating Original Research Article
Pages 352-363
Richard Paylor, Nardos Lijam, Michael P. McDonald, Jacqueline N. Crawley, Daniel J. Sussman, Anthony Wynshaw-Boris

Chapter 3.1.10 Targeting genes associated with mammalian behavior: past mistakes and future solutions Original Research Article
Pages 364-375
Robert Gerlai

Chapter 3.2.1 Gene transfer and therapy in the CNS Original Research Article
Pages 379-395
Marie-Claude Senut, Steven T. Suhr, Fred H. Gage

Chapter 3.2.2 Adenovirus vectors for gene transfer into the central nervous system Original Research Article
Pages 396-414
Martine Barkats, Olga Corti, Jacques Mallet

Chapter 3.2.3 Regulatable adenoviral technology in behavioural neuroscience Original Research Article
Pages 415-424
E. Clea Warburton, Brad Geddes, Thomas Harding, James B. Uney

Chapter 3.2.4 Antisense oligonucleotides to selectively suppress gene expression in the brain Original Research Article
Pages 425-437
Giuseppe Pollio, Adriana Maggi

Chapter 3.2.5 Application of recombinant proteins, peptides and antibodies in exploring the role of Src in regulating synaptic function Original Research Article
Pages 438-454
Michael W. Salter

Chapter 3.2.6 The use of immunoadhesins in neurobiology Original Research Article
Pages 455-464
David L. Shelton

Chapter 3.2.7 Protein targeting in the functional analysis of EphA receptors: the use of immunoadhesins Original Research Article
Pages 465-484
R. Gerlai, N. Shinsky, A. Shih, P. Williams, J. Winer, M. Armanini, P. Moran, B. Cairns, J. Winslow, W.-Q. Gao, H.S. Phillips

Chapter 3.3.1 A novel approach to Drosophila neurophysiology” the targeted expression of aequorin Original Research Article
Pages 487-495
Philippe Rosay, Kim Kaiser, J. Douglas Armstrong

Chapter 3.3.2 Behavior-genetic and molecular analysis of naturally occurring variation in Drosophila larval foraging behavior Original Research Article
Pages 496-511
Marla B. Sokolowski, Craig A.L. Riedl

Chapter 3.3.3 Structure-function analysis of the Drosophila optic lobes Original Research Article
Pages 512-536
Gert O. Pflugfelder

Chapter 3.3.4 Testing associative learning in Drosophila Original Research Article
Pages 537-550
Thomas Préat

Chapter 3.3.5 Caenorhabditis elegans and the genetics of learning Original Research Article
Pages 551-568
Kevin R. Peters, Jennifer A. Galloway, Catharine H. Rankin

Chapter 3.3.6 Forward genetic approaches in the analysis of Caenorhabditis elegans Original Research Article
Pages 569-584
Anne C. Hart

Chapter 3.3.7 Analyzing neuropeptide function in Caenorhabditis elegans by reverse genetics Original Research Article
Pages 585-601
Chris Li

Chapter 4.1 Ethological approaches in behavioral neurogenetic research Original Research Article
Pages 605-613
Robert Gerlai

Chapter 4.2 What animals remember about past events: an ethological approach Original Research Article
Pages 614-626
Nicola S. Clayton

Chapter 4.3 Motor performance of spontaneous murine mutations with cerebellar atrophy Original Research Article
Pages 627-637
R. Lalonde, C. Strazielle

Chapter 4.4 Methodological considerations for testing learning in mice Original Research Article
Pages 638-651
Wim E. Crusio

Chapter 4.5 Drug and alcohol dependence-related behaviors Original Research Article
Pages 652-666
John C. Crabbe, Christopher L. Cunningham

Chapter 4.6 Evaluating anxiety in rodents Original Research Article
Pages 667-673
Jacqueline N. Crawley

Chapter 4.7 A neurobehavioral system approach in rats to study the molecular biology of fear Original Research Article
Pages 674-695
Jeffrey B. Rosen, Seema Malkani, Karin J. Wallace, Barbara L. Thompson

Chapter 4.8 Measuring aggression in the mouse Original Research Article
Pages 696-709
Pierre L. Roubertoux, Isabelle Le Roy, Stephane Mortaud, Fernando Perez-Diaz, Sylvie Tordjman

Chapter 4.9 Methodological issues in the assessment of behavioral development in laboratory mice Original Research Article
Pages 710-721
P.E. Wainwright

Chapter 4.10 Understanding maternal behavior: analyses of behavior, c-Fos expression and calmodulin binding proteins in the medial preoptic area and other areas of the rat brain Original Research Article
Pages 722-737
Alison S. Fleming, Danton H. O’Day

Chapter 4.11 Measuring rodent exploratory behavior Original Research Article
Pages 738-749
Catherine Belzung

Chapter 5.1 sychiatric genetics current perspective Original Research Article
Pages 753-772
Douglas F. Levinson

Chapter 5.2 Defining phenotypes for psychiatric genetics Original Research Article
Pages 773-782
Marika Nosten-Bertrand, Frank Bellivier, Marion Leboyer

Chapter 5.3 Trinucleotide repeat disorders Original Research Article
Pages 783-804
Gunnar Sandberg, Kerstin Lindblad, Ben A. Oostra, Martin Schalling

CHAPTER 5.4 Finding liability genes for schizophrenia Original Research Article
Pages 805-819
Nick J.O. Jacobsen, Nigel M. Williams, Michael J. Owen

CHAPTER 5.5 Genetics of idiopathic epilepsy Original Research Article
Pages 820-840
Louise Bate, Mark Gardiner

CHAPTER 5.6 Identification and functional analysis of genes and genetic risk factors in Alzheimer’s disease Original Research Article
Pages 841-862
Chris De Jonghe, Christine Van Broeckhoven

Chapter 5.7 Aging, Alzheimer’s disease and frameshift mutations Original Research Article
Pages 863-879
Wilhelmina H. Van den Hurk, Fred W. Van Leeuwen, Gerard J.M. Martens

Chapter 5.8 Transgenic mouse models of Alzheimer’s disease Original Research Article
Pages 880-894
Karen Duff

Chapter 5.9 Modelling Down syndrome in mice Original Research Article
Pages 895-913
Mara Dierssen, Melanie Pritchard, Cristina Fillat, Mariona Arbonés, Josep M. Aran, Jesfls Flórez, Estivill Xavier

Chapter 6.1 Genes and human behavior: scientific and ethicalimplications of the human genome project Original Research Article
Pages 917-926
Jon Beckwith

Chapter 6.2 Ethical issues and psychiatric genetics Original Research Article
Pages 927-935
A.E. Farmer, P. McGuffin

Chapter 6.3 Ethical implications of knock-out and transgenesistechniques for animal research Original Research Article
Pages 936-948
Louis Marie Houdebine

Subject Index
Pages 949-965

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