Bioaffinity Chromatography

Jaroslava Turková (Eds.)0444890300, 9780444890306

Bioaffinity chromatography is now the preferred choice for the purification, determination or removal of many biologically active substances. This text includes information on biologically active substances with their affinants, solid supports and methods of coupling, summarized in tables covering classical, high-performance liquid and large-scale bioaffinity chromatography. Optimization of the preparation and the use of highly active and stable biospecific adsorbents is discussed in several chapters. Following a chapter dealing with the choice of affinity ligands, affinity-sorbent bonding is described in detail. Other chapters give information on solid supports, the most common coupling procedures and a general discussion of sorption and elution. Several applications of bioaffinity chromatography are described, such as quantitative evaluation of biospecific complexes and many uses in medicine and in the biotechnology industry.

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Table of contents :
Content:
Edited by
Page iii

Copyright page
Page iv

Preface
Pages xvii-xviii
Jaroslava Turková

Chapter 1 Introduction
Pages 1-7

Chapter 2 The Principle, History and use of Bioaffinity Chromatography
Pages 9-30

Chapter 3 Choice of Affinity Ligands (Affinants)
Pages 31-111

Chapter 4 General Considerations on Affinant – Sorbent Bonding
Pages 113-169

Chapter 5 Solid Matrix Supports
Pages 171-214

Chapter 6 Survey of the Most Common Coupling Procedures
Pages 215-281

Chapter 7 Characterization of Supports and Immobilized Affinity Ligands
Pages 283-312

Chapter 8 General Considerations on Sorption, Elution and Non-Specific Binding
Pages 313-370

Chapter 9 Bioaffinity Chromatography in the Isolation, Determination or Removal of Biologically Active Substances
Pages 371-641

Chapter 10 Immobilization of Enzymes by Biospecific Adsorption to Immobilized Monoclonal or Polyclonal Antibodies
Pages 643-655

Chapter 11 Study of the Modification, Mechanism of Action and Structure of Biologically Active Substances Using Bioaffinity Chromatography
Pages 657-672

Chapter 12 Solid-Phase Immunoassay and Enzyme-Linked Lectin Assay
Pages 673-701

Chapter 13 Several Examples of the Application of Biospecific Adsorption in Medicine
Pages 703-730

Chapter 14 Application of Bioaffinity Chromatography to the Quantitative Evaluation of Specific Complexes
Pages 731-760

Chapter 15 Theory of Bioaffinity Chromatography
Pages 761-780

Subject Index
Pages 781-796

Journal of Chromatography Library
Pages 797-800