High Throughput Bioanalytical Sample Preparation: Methods and Automation Strategies

David A. Wells (Eds.)9780444510297, 044451029X

High Throughput Bioanalytical Sample Preparation: Methods and Automation Strategies is an authoritative reference on the current state-of-the-art in sample preparation techniques for bioanalysis. This book focuses on high throughput (rapid productivity) techniques and describes exactly how to perform and automate these methodologies, including useful strategies for method development and optimization. A thorough review of the literature is included within each of these chapters describing high throughput sample preparation techniques: protein removal by precipitation; equilibrium dialysis and ultrafiltration; liquid-liquid extraction; solid-phase extraction; and various on-line techniques.The text begins with an introductory overview of the role of bioanalysis in pharmaceutical drug development. Fundamental understanding of the strategies for sample preparation is reinforced next, along with essential concepts in extraction chemistry. Several chapters introduce and discuss microplates, accessory products and automation devices. Particular strategies for efficient use of automation within a bioanalytical laboratory are also presented. The subject material then reviews protein precipitation, liquid-liquid extraction, solid-phase extraction and various on-line sample preparation approaches. The book concludes with information on recent advances in sample preparation, such as solid-phase extraction in a card format and higher density extraction plates.Important objectives that can be accomplished when the strategies presented in this book are followed include: improved efficiency in moving discovery compounds to preclinical status with robust analyticalmethods; return on investment in automation for sample preparation; and improved knowledge and expertise of laboratory staff.

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Table of contents :
Content:
Preface
Pages v-vi
David A. Wells

List of acronyms
Pages xxvii-xxx

Chapter 1 Role of bioanalysis in pharmaceutical drug development Original Research Article
Pages 1-40

Chapter 2 Fundamental strategies for bioanalytical sample preparation Original Research Article
Pages 41-74

Chapter 3 High throughput tools for bioanalysis: Microplates Original Research Article
Pages 75-105

Chapter 4 High throughput tools for bioanalysis: Accessory products Original Research Article
Pages 107-133

Chapter 5 Automation tools and strategies for bioanalysis Original Research Article
Pages 135-197

Chapter 6 Protein precipitation: High throughput techniques and strategies for method development Original Research Article
Pages 199-254

Chapter 7 Protein precipitation: Automation strategies Original Research Article
Pages 255-276

Chapter 8 Liquid-liquid extraction: High throughput techniques Original Research Article
Pages 277-306

Chapter 9 Liquid-liquid extraction: Strategies for method development and optimization Original Research Article
Pages 307-326

Chapter 10 Liquid-liquid extraction: Automation strategies Original Research Article
Pages 327-360

Chapter 11 Solid-phase extraction: High throughput techniques Original Research Article
Pages 361-432

Chapter 12 Solid-phase extraction: Strategies for method development and optimization Original Research Article
Pages 433-484

Chapter 13 Solid-phase extraction: Automation strategies Original Research Article
Pages 485-504

Chapter 14 On-line sample preparation: High throughput techniques and strategies for method development Original Research Article
Pages 505-573
Jing-Tao Wu, David A.Wells

Chapter 15 Advances in sample preparation for bioanalysis Original Research Article
Pages 575-588

Appendix I: Microplate worksheets
Pages 589-592

Appendix II: List of vendors Original Research Article
Pages 593-598

Subject index
Pages 599-610