Protein Folding Kinetics Biophysical Methods

Bengt Nolting3540657436, 9783540657439

The book presents modern biophysical methods, in particular: 1. Equipment of rapid reaction kinetics. 2. Novel methods of the high structural resolution of protein folding reactions with emphasis on protein engineering approaches. 3. Mathematical treatment of a large variety of common types of chemical or physical reactions. 4. Experimental techniques for the resolution of chemical reactions and structural changes of proteins. Important techniques include DNA cloning and site-directed mutagenesis, Phi-value analysis, NMR, temperature jumping, fluorescence, circular dichroism, and pulsed LASER spectroscopy, continuous-flow and stopped-flow methods, ultrafast mixing, labeling methods, pressure jump and repetitive pressure perturbation, sound velocity measurement, and dielectric relaxation. 145 figures and 15 tables provide essential information of experimental details not easily found elsewhere.